Article
Preferential binding of hot spot mutant p53 proteins to supercoiled DNA in vitro and in cells.
PloS one - 1 Jan 2013
Brázdová Marie, Navrátilová Lucie, Tichý Vlastimil, Němcová Kateřina, Lexa Matej, Hrstka Roman, Pečinka Petr, Adámik Matej, Vojtesek Borivoj, Paleček Emil, Deppert Wolfgang, Fojta Miroslav
Abstract excerpt
Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53 to DNA is assumed to be involved in mutp53-mediated repression or activation of several mutp53 target genes. To investigate the importance of DNA topology on mutp53-DNA recognition in vitro and in cells, we analyzed the interaction of seven hot spot mutp53 proteins with topologically different DNA substrates...
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