Article
Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual Staining.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Zhong Sheng, Wang Zhijuan, Qu Li-Jia
Abstract excerpt
Mutant phenotype observation is the most useful and important method to study which biological process a gene-of-interest is involved in. In flowering plants, excessive pollen grains land and germinate on the stigma, then pollen tubes grow through the transmitting tract to reach the ovules, eventually enter the micropyle to complete double fertilization. First, for mutants whose homozygotes could not be obtained...
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