Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimized method for growing in vitro Arabidopsis thaliana pollen tubes.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Borassi Cecilia, Di Giorgio Juliana Pérez, Scarpin María R, Muschietti Jorge, Estevez José M

Abstract excerpt

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arabidopsis
Share to Arabidopsis Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cell Polarity

Share to DNA, Bacterial

Share to Gene Expression Regulation, Developmental

Share to Gene Expression Regulation, Plant

Share to Germination

Share to Pollen Tube

Source

Identifiers

PMID: 25408441
DOI: 10.1007/978-1-4939-1902-4_3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimized method for growing in vitro Arabidopsis thaliana pollen tubes.PMID 25408441Read ↗

Pollen tube development.Semantic proximity Read ↗

Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual Staining.Semantic proximity Read ↗

Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil.Semantic proximity Read ↗

Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana.Semantic proximity Read ↗

Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex.Semantic proximity Read ↗

A Toolkit for Teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thaliana.Semantic proximity Read ↗

Vacuolar and ER-Ca2+-ATPases regulate calcium dynamics during pollen tube growth in Arabidopsis thaliana.Semantic proximity Read ↗

A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes.Semantic proximity Read ↗

Two Arabidopsis AGC kinases are critical for the polarized growth of pollen tubes.Semantic proximity Read ↗

A land plant-specific VPS13 mediates polarized vesicle trafficking in germinating pollen.Semantic proximity Read ↗

Control of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to tip-localized calcium influx.Semantic proximity Read ↗

TipChip: a modular, MEMS-based platform for experimentation and phenotyping of tip-growing cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.