Article
Molecular characterization of extraintestinal Escherichia coli isolates in Japan: relationship between sequence types and mutation patterns of quinolone resistance-determining regions analyzed by pyrosequencing.
Journal of clinical microbiology - 1 Jun 2013
Aoike Nozomi, Saga Tomoo, Sakata Ryuji, Yoshizumi Ayumi, Kimura Soichiro, Iwata Morihiro, Yoshizawa Sadako, Sugasawa Yasuyuki, Ishii Yoshikazu, Yamaguchi Keizo, Tateda Kazuhiro
Abstract excerpt
Infection from fluoroquinolone-resistant Enterobacteriaceae is an increasing health problem worldwide. In the present study, we developed a pyrosequencing-based high-throughput method for analyzing the nucleotide sequence of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. By using this method, we successfully determined the QRDR sequences of 139 out of 140 clinical Escherichia coli...
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