Article
In vivo phosphorylation dynamics of the Bordetella pertussis virulence-controlling response regulator BvgA.
Molecular microbiology - 1 Apr 2013
Boulanger Alice, Chen Qing, Hinton Deborah M, Stibitz Scott
Abstract excerpt
We have used protein electrophoresis through polyacrylamide gels derivatized with the proprietary ligand Phos-tag™ to separate the response regulator BvgA from its phosphorylated counterpart BvgA∼P. This approach has allowed us to readily ascertain the degree of phosphorylation of BvgA in in vitro reactions, or in crude lysates of Bordetella pertussis grown under varying laboratory conditions. We have used this...
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