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Article

The effect of forced growth of cells into 3D spheres using low attachment surfaces on the acquisition of stemness properties.

Biomaterials - 1 Apr 2013

Su Guannan, Zhao Yannan, Wei Jianshu, Han Jin, Chen Lei, Xiao Zhifeng, Chen Bing, Dai Jianwu

Abstract excerpt

Embryonic stem cells (ESCs) and neural progenitor cells form three-dimensional (3D) colonies or spheres in vitro, and 3D sphere is reported to help maintaining the stemness of stem cells, but the effect of 3D sphere formation on cell reprogramming remains unknown. Here we examined whether 3D sphere culture have any impact on the differentiated cells. We cultured bladder cancer cell RT4 and non-cancerous cell...

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Identifiers

PMID: 23439133
DOI: 10.1016/j.biomaterials.2013.01.044

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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