Article
Studies on enzymatic activity and conformational stability of muscle acylphosphatase mutated at conserved lysine residues.
Protein engineering - 1 Jul 1998
Chiti F, Magherini F, Taddei N, Ilardi C, Stefani M, Bucciantini M, Dobson C M, Ramponi G
Abstract excerpt
An oligonucleotide-directed mutagenesis study was carried out on the five acylphosphatase conserved lysine residues to assess their possible participation in enzyme active site formation and their contribution to the enzyme conformational stability. The study was designed to eliminate the ambigui...
Topics
- Acid Anhydride Hydrolases
- Animals
- Conserved Sequence
- Kinetics
- Muscle, Skeletal
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- Acylphosphatase