Article
Rapid and non-enzymatic in vitro retrieval of tumour cells from surgical specimens.
PloS one - 1 Jan 2013
Mack Brigitte, Eggert Carola, Eder Katharina, Imrich Sannia, Baumeister Philipp, Harréus Ulrich, Gires Olivier
Abstract excerpt
The study of tumourigenesis commonly involves the use of established cell lines or single cell suspensions of primary tumours. Standard methods for the generation of short-term tumour cell cultures include the disintegration of tissue based on enzymatic and mechanical stress. Here, we describe a simple and rapid method for the preparation of single cells from primary carcinomas, which is independent of enzymatic...
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