Article
Identification of the human liver cytochrome P-450 responsible for coumarin 7-hydroxylase activity.
The Biochemical journal - 15 Apr 1990
Miles J S, McLaren A W, Forrester L M, Glancey M J, Lang M A, Wolf C R
Abstract excerpt
1. We have constructed a full-length human liver cytochrome P450IIA cDNA from a partial-length clone by oligonucleotide-directed mutagenesis, and subcloned it into the monkey kidney (COS-7) cell expression vector, pSVL. 2. The cDNA encodes a 49 kDa protein with coumarin 7-hydroxylase (COH) activity which cross-reacts with antisera to the mouse cytochrome P-450 isoenzyme responsible for COH activity and comigrates...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.