Identification of the human liver cytochrome P-450 responsible for coumarin 7-hydroxylase activity.

doi:10.1042/bj2670365

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Article

Identification of the human liver cytochrome P-450 responsible for coumarin 7-hydroxylase activity.

The Biochemical journal - 15 Apr 1990

Miles J S, McLaren A W, Forrester L M, Glancey M J, Lang M A, Wolf C R

Abstract excerpt

1. We have constructed a full-length human liver cytochrome P450IIA cDNA from a partial-length clone by oligonucleotide-directed mutagenesis, and subcloned it into the monkey kidney (COS-7) cell expression vector, pSVL. 2. The cDNA encodes a 49 kDa protein with coumarin 7-hydroxylase (COH) activity which cross-reacts with antisera to the mouse cytochrome P-450 isoenzyme responsible for COH activity and comigrates...

Topics

Amino Acid Sequence
Animals
Aryl Hydrocarbon Hydroxylases
Base Sequence
Cell Line
Cytochrome P-450 CYP2A6
Cytochrome P-450 Enzyme System
DNA
Humans
Liver
Mixed Function Oxygenases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Molecular Weight

Mutation

Oligonucleotide Probes

Sequence Homology, Nucleic Acid

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 2334398
DOI: 10.1042/bj2670365
PMCID: PMC1131297

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2334398-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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