Article
Identification and functional characterization of the Lactococcus lactis rfb operon, required for dTDP-rhamnose Biosynthesis.
Journal of bacteriology - 1 Mar 2004
Boels Ingeborg C, Beerthuyzen Marke M, Kosters Marit H W, Van Kaauwen Martijn P W, Kleerebezem Michiel, De Vos Willem M
Abstract excerpt
dTDP-rhamnose is an important precursor of cell wall polysaccharides and rhamnose-containing exopolysaccharides (EPS) in Lactococcus lactis. We cloned the rfbACBD operon from L. lactis MG1363, which comprises four genes involved in dTDP-rhamnose biosynthesis. When expressed in Escherichia coli, the lactococcal rfbACBD genes could sustain heterologous production of the Shigella flexneri O antigen, providing...
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