Article
Quantification of in vivo oxidative damage in Caenorhabditis elegans during aging by endogenous F3-isoprostane measurement.
Aging cell - 1 Apr 2013
Labuschagne Christiaan F, Stigter Edwin C A, Hendriks Margriet M W B, Berger Ruud, Rokach Joshua, Korswagen Hendrik C, Brenkman Arjan B
Abstract excerpt
Oxidative damage is thought to be a major cause in development of pathologies and aging. However, quantification of oxidative damage is methodologically difficult. Here, we present a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach for accurate, sensitive, and linear in vivo quantification of endogenous oxidative damage in the nematode Caenorhabditis elegans, based on F3-isoprostanes....
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