Article
Estimation of the deamidation rates of major deamidation sites in a Fab fragment of mouse IgG1-κ by capillary isoelectric focusing of mutated Fab fragments.
Analytical chemistry - 5 Feb 2013
Shimura Kiyohito, Hoshino Makoto, Kamiya Keiichiro, Enomoto Manabu, Hisada Sunao, Matsumoto Hiroyuki, Novotny Mark, Kasai Ken-ichi
Abstract excerpt
The deamidation of asparagine (Asn or N) residues in proteins is a common post-translational chemical modification. The identification of deamidation sites and determination of the degree of deamidation have been carried out by the combination of peptide mapping and mass spectrometry. However, when a peptide fragment contains multiple amides, such analysis becomes difficult and sometimes impossible. In this...
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