Article
Hierarchical binding of DNA fragments derived from scaffold-attached regions: correlation of properties in vitro and function in vivo.
Biochemistry - 14 Aug 1990
Mielke C, Kohwi Y, Kohwi-Shigematsu T, Bode J
Abstract excerpt
On its upstream side, the human interferon-beta gene is flanked by a 7-kb SAR (scaffold-attached region) DNA element. The core of this element is determined and subjected to in vitro reassociations with isolated scaffolds. Binding properties of SAR fragments with decreasing length are quantified...
Topics
- Animals
- Base Composition
- Base Sequence
- Cells, Cultured
- DNA
- DNA-Binding Proteins
- Gene Expression Regulation
- Genetic Markers
- Humans
- Interferon Type I
- Mice
- Molecular Sequence Data
- Mutation
- Regulatory Sequences, Nucleic Acid
- Transcription, Genetic