Article
[Antigenic comparative analysis of Newcastle disease viruses with evolutional mutations in HN and F genes under antibody immune pressures].
Bing du xue bao = Chinese journal of virology - 1 Sept 2012
He Yu-Ting, Gong Yan-Yan, Zhao Peng, Cui Zhi-Zhong
Abstract excerpt
In chicken fibroblast cell (CEF) cultures with antiserum against Newcastle disease virus (NDV) strain TZ060107, the virus was passed serially for 50 passages in 3 independent lineages. HN and F genes were amplified and sequenced every 10 passages. The derived virus A1-50 with most mutations among 3 lineages was further passed for another 50 passages in CEF with or without antiserum against A1-50, each in 3...
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