High-resolution transcriptome and genome-wide dynamics of RNA polymerase and NusA in Mycobacterium tuberculosis

Abstract excerpt

To construct a regulatory map of the genome of the human pathogen, Mycobacterium tuberculosis, we applied two complementary high-resolution approaches: strand-specific RNA-seq, to survey the global transcriptome, and ChIP-seq, to monitor the genome-wide dynamics of RNA polymerase (RNAP) and the anti-terminator NusA. Although NusA does not bind directly to DNA, but rather to RNAP and/or to the nascent transcript,...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacterial Genetics and Biotechnology
• Share to RNA and protein synthesis mechanisms
• Share to Tuberculosis Research and Epidemiology