Article
Identification of novel mutant p53 interacting proteins by proteomic analysis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Deb Sumitra, Graves Paul R
Abstract excerpt
Protein-protein interaction studies can provide valuable insight into protein function. One of the most practical and high-yielding approaches is immunoprecipitation of a bait protein followed by mass spectrometry to identify co-precipitating proteins. Here we describe an effective and simplified version of this method that can be performed in most laboratories using standard laboratory equipment (apart from the...
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