Article
A comprehensive transcriptome assembly of Pigeonpea (Cajanus cajan L.) using sanger and second-generation sequencing platforms.
Molecular plant - 1 Sept 2012
Kudapa Himabindu, Bharti Arvind K, Cannon Steven B, Farmer Andrew D, Mulaosmanovic Benjamin, Kramer Robin, Bohra Abhishek, Weeks Nathan T, Crow John A, Tuteja Reetu, Shah Trushar, Dutta Sutapa, Gupta Deepak K, Singh Archana, Gaikwad Kishor, Sharma Tilak R, May Gregory D, Singh Nagendra K, Varshney Rajeev K
Abstract excerpt
A comprehensive transcriptome assembly for pigeonpea has been developed by analyzing 128.9 million short Illumina GA IIx single end reads, 2.19 million single end FLX/454 reads, and 18 353 Sanger expressed sequenced tags from more than 16 genotypes. The resultant transcriptome assembly, referred to as CcTA v2, comprised 21 434 transcript assembly contigs (TACs) with an N50 of 1510 bp, the largest one being ~8 kb....
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