Article
[Establishment of a large-scale bi-directional sequencing and genotyping platform for MICA gene exons 2 to 4].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Oct 2012
Gao Su-qing, Deng Zhi-hui, Xu Yun-ping, Wang Da-ming, He Liu-mei, Jin Shi-zheng
Abstract excerpt
OBJECTIVE: To establish a stable and large-scale bi-directional sequencing platform for genotyping MICA gene exons 2 to 4, and to analyze single nucleotide polymorphisms(SNP) of the region. METHODS: Primers for particular alleles of MICA gene exons 2 to 5 were designed. Optimal conditions for PCR amplification and sequencing reaction were explored. A commercialized one-way sequencing kit for MICA allele was used...
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