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Article

Identification of a single base-pair mutation of TAA (Stop codon) → GAA (Glu) that causes light chain extension in a CHO cell derived IgG1.

mAbs - 1 Jan 2000

Zhang Taylor, Huang Yungfu, Chamberlain Scott, Romeo Tony, Zhu-Shimoni Judith, Hewitt Daniel, Zhu Mary, Katta Viswanatham, Mauger Brad, Kao Yung-Hsiang

Abstract excerpt

We describe here the identification of a stop codon TAA (Stop) → GAA (Glu) = Stop221E mutation on the light chain of a recombinant IgG1 antibody expressed in a Chinese hamster ovary (CHO) cell line. The extended light chain variants, which were caused by translation beyond the mutated stop codon to the next alternative in-frame stop codon, were observed by mass spectra analysis. The abnormal peptide peaks present...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 23018810
DOI: 10.4161/mabs.22232
PMCID: PMC3502236

Genes

GAA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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