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Article

[Establishment of optimized neuronal differentiation-promoting model derived from P19 embryonic carcinoma cells].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 1 Jul 2012

Mei Yu-qin, Liang Xing-guang, Lou Yi-jia, Yu Yong-ping, Zhu Dan-yan

Abstract excerpt

OBJECTIVE: To establish an optimized primary drug screen model of neuronal differentiation using P19 embryonal carcinoma cells. METHODS: The final concentration of retinoid acid (RA), days of suspension culture, manner of adherent culture, suitable cell density and adherent culture medium were tested, respectively. Two stages of neuronal differentiation were examined based on morphological changes and...

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Identifiers

PMID: 22927072
DOI: 10.3785/j.issn.1008-9292.2012.04.005

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