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Article

Enhancement of the substrate scope of transketolase.

Chembiochem : a European journal of chemical biology - 3 Sept 2012

Ranoux Adeline, Karmee Sanjib K, Jin Jianfeng, Bhaduri Anirban, Caiazzo Aldo, Arends Isabel W C E, Hanefeld Ulf

Abstract excerpt

To enhance the activity of transketolase towards nonphosphorylated substrates and enlarge the scope of its substrates, notably to long polyol aldehyde acceptors (D-ribose or D-glucose), a rational design-supported evolution strategy was applied. By using docking experiments, an in silico library, and iterative mutagenesis, libraries of single- and double-point mutants were designed and generated. A...

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Identifiers

PMID: 22821820
DOI: 10.1002/cbic.201200240

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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