Article
High-throughput multilocus sequence typing: bringing molecular typing to the next level.
PloS one - 1 Jan 2012
Boers Stefan A, van der Reijden Wil A, Jansen Ruud
Abstract excerpt
Multilocus sequence typing (MLST) is a widely used system for typing microorganisms by sequence analysis of housekeeping genes. The main advantage of MLST in comparison to other typing techniques is the unambiguity and transferability of sequence data. However, a main disadvantage is the high cost of DNA sequencing. Here we introduce a high-throughput MLST (HiMLST) method that employs next-generation sequencing...
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