Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac-related small GTP-binding protein fail to develop necrotic lesions upon elicitor infiltration.

Abstract excerpt

Using an RT-PCR approach a rac-related cDNA clone, designated Ms-rac1, was isolated from Medicago sativa (alfalfa). Ms-rac1 encodes a putative protein of 197 amino acids, which is closely related to known Rac-related GTP-binding proteins from Pisum sativum and Arabidopsis thaliana. RT-PCR analysis demonstrated that Ms-rac1 is ubiquitously expressed in various tissues in alfalfa. Expression of Ms-rac1 in...

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