Article
Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac-related small GTP-binding protein fail to develop necrotic lesions upon elicitor infiltration.
Molecular & general genetics : MGG - 1 Jun 2000
Schiene K, Pühler A, Niehaus K
Abstract excerpt
Using an RT-PCR approach a rac-related cDNA clone, designated Ms-rac1, was isolated from Medicago sativa (alfalfa). Ms-rac1 encodes a putative protein of 197 amino acids, which is closely related to known Rac-related GTP-binding proteins from Pisum sativum and Arabidopsis thaliana. RT-PCR analysis demonstrated that Ms-rac1 is ubiquitously expressed in various tissues in alfalfa. Expression of Ms-rac1 in...
Topics
- Amino Acid Sequence
- Arabidopsis
- Base Sequence
- DNA Primers
- DNA, Antisense
- DNA, Complementary
- DNA, Plant
- Gene Expression
- Medicago sativa
- Molecular Sequence Data
- Necrosis
- Pisum sativum
- Phenotype