Article
Specific truncations of an acetolactate synthase gene from Brassica napus efficiently complement ilvB/ilvG mutants of Salmonella typhimurium.
Molecular & general genetics : MGG - 1 Oct 1990
Wiersma P A, Hachey J E, Crosby W L, Moloney M M
Abstract excerpt
The expression of an acetolactate synthase (ALS) gene isolated from the cruciferous plant Brassica napus was investigated in Salmonella typhimurium. Using an expression plasmid containing the highly active trc (trp-lac) promoter, several plant ALS constructs were made containing successive in-frame truncations from the 5' end of the coding region. Functional complementation by these plant ALS constructs of a S....
Topics
- Acetolactate Synthase
- Amino Acid Sequence
- Base Sequence
- Brassica
- Cloning, Molecular
- Drug Resistance
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Genes, Plant
- Genetic Complementation Test