Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

1 Jan 2012

Abstract excerpt

BACKGROUND: Yeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns about strain stability of P. pastoris diploids. The aim of the study reported here is to investigate heterologous protein expression in diploid P. pastoris strains and to evaluate diploid strain...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Fungal and yeast genetics research
Share to Monoclonal and Polyclonal Antibodies Research
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 22748191
DOI: 10.1186/1475-2859-11-91

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein productionPMID 22748191Read ↗

High-throughput screening and selection of yeast cell lines expressing monoclonal antibodiesSemantic proximity Read ↗

Construction of a Large Synthetic Human Fab Antibody Library on Yeast Cell Surface by Optimized Yeast MatingSemantic proximity Read ↗

Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments.Semantic proximity Read ↗

Yeast-based production platform for potent and stable heavy chain-only antibodiesSemantic proximity Read ↗

Comparative genomics and transcriptomics of Pichia pastoris.Semantic proximity Read ↗

An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastorisSemantic proximity Read ↗

Reexamining opportunities for therapeutic protein production in eukaryotic microorganismsSemantic proximity Read ↗

Classical Genetics of Pichia pastoris.Semantic proximity Read ↗

Mating antibody phage display with proteomicsSemantic proximity Read ↗

Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast matingSemantic proximity Read ↗

High‐Level Expression of Recombinant IgG in the Human Cell Line PER.C6Semantic proximity Read ↗

Engineering of Immunoglobulin Fc Heterodimers Using Yeast Surface-Displayed Combinatorial Fc Library Screening.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.