Article
Construction and manipulation of a new Kaposi's sarcoma-associated herpesvirus bacterial artificial chromosome clone.
Journal of virology - 1 Sept 2012
Brulois Kevin F, Chang Heesoon, Lee Amy Si-Ying, Ensser Armin, Wong Lai-Yee, Toth Zsolt, Lee Sun Hwa, Lee Hye-Ra, Myoung Jinjong, Ganem Don, Oh Tae-Kwang, Kim Jihyun F, Gao Shou-Jiang, Jung Jae U
Abstract excerpt
Efficient genetic modification of herpesviruses such as Kaposi's sarcoma-associated herpesvirus (KSHV) has come to rely on bacterial artificial chromosome (BAC) technology. In order to facilitate this approach, we generated a new KSHV BAC clone, called BAC16, derived from the rKSHV.219 virus, which stems from KSHV and Epstein-Barr virus-coinfected JSC1 primary effusion lymphoma (PEL) cells. Restriction enzyme and...
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