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Killer cell immunoglobulin-like receptors (KIR) typing by DNA sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Hou Lihua, Chen Minghua, Steiner Noriko, Kariyawasam Kanthi, Ng Jennifer, Hurley Carolyn K

Abstract excerpt

DNA sequencing is a powerful technique for identifying allelic variation within the natural killer cell immunoglobulin-like receptor genes. Because of the relatively large size of the KIR genes, each locus is amplified in two or more overlapping segments. Sanger sequencing of each gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a...

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PMID: 22665249
DOI: 10.1007/978-1-61779-842-9_25
PMCID: PMC3478768

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