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Assessment of the stochastic threshold, back- and forward stutter filters and low template techniques for NGM.

Forensic science international. Genetics - 1 Dec 2012

Westen Antoinette A, Grol Laurens J W, Harteveld Joyce, Matai Anuska S, de Knijff Peter, Sijen Titia

Abstract excerpt

The AmpFlSTR(®) NGM™ kit shows an increased sensitivity compared to previous AmpFlSTR(®) kits, and the addition of a 29th PCR cycle was found to be the major cause for this. During in-house validation, we evaluated whether the increased sensitivity requires elevation of the stochastic threshold (below which alleles are prone to drop out due to low template amplification effects). To determine the stochastic...

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PMID: 22633964
DOI: 10.1016/j.fsigen.2012.05.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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