Article
Isolation of Plasmodium falciparum by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples.
Malaria journal - 14 May 2012
Boissière Anne, Arnathau Céline, Duperray Christophe, Berry Laurence, Lachaud Laurence, Renaud François, Durand Patrick, Prugnolle Franck
Abstract excerpt
BACKGROUND: Flow cytometry and cell sorting are powerful tools enabling the selection of particular cell types within heterogeneous cell mixtures. These techniques, combined with whole genome amplification that non-specifically amplify small amounts of starting DNA, offer exciting new opportunities for the study of malaria genetics. Among them, two are tested in this paper: (1) single cell genotyping and (2)...
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