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Article

Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants.

Applied and environmental microbiology - 1 Jul 2012

Farkas Joel, Stirrett Karen, Lipscomb Gina L, Nixon William, Scott Robert A, Adams Michael W W, Westpheling Janet

Abstract excerpt

We recently reported the isolation of a mutant of Pyrococcus furiosus, COM1, that is naturally and efficiently competent for DNA uptake. While we do not know the exact nature of this mutation, the combined transformation and recombination frequencies of this strain allow marker replacement by direct selection using linear DNA. In testing the limits of its recombination efficiency, we discovered that marker...

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Identifiers

PMID: 22544252
DOI: 10.1128/AEM.00936-12
PMCID: PMC3370475

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Source receipt: pubmed, retrieved 7 Sept 2026.

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