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Article

Targeted single nucleotide polymorphism (SNP) discovery in a highly polyploid plant species using 454 sequencing.

Plant biotechnology journal - 1 May 2009

Bundock Peter C, Eliott Frances G, Ablett Gary, Benson Adam D, Casu Rosanne E, Aitken Karen S, Henry Robert J

Abstract excerpt

Discovering single nucleotide polymorphisms (SNPs) in specific genes in a heterozygous polyploid plant species, such as sugarcane, is challenging because of the presence of a large number of homologues. To discover SNPs for mapping genes of interest, 454 sequencing of 307 polymerase chain reaction (PCR) amplicons (> 59 kb of sequence) was undertaken. One region of a four-gasket sequencing run, on a 454 Genome...

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PMID: 19386042
DOI: 10.1111/j.1467-7652.2009.00401.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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