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Article

Biotinylation of lysine method identifies acetylated histone H3 lysine 79 in Saccharomyces cerevisiae as a substrate for Sir2.

Proceedings of the National Academy of Sciences of the United States of America - 17 Apr 2012

Bheda Poonam, Swatkoski Stephen, Fiedler Katherine L, Boeke Jef D, Cotter Robert J, Wolberger Cynthia

Abstract excerpt

Although the biological roles of many members of the sirtuin family of lysine deacetylases have been well characterized, a broader understanding of their role in biology is limited by the challenges in identifying new substrates. We present here an in vitro method that combines biotinylation and mass spectrometry (MS) to identify substrates deacetylated by sirtuins. The method permits labeling of deacetylated...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Silent Information Regulator Proteins, Saccharomyces cerevisiae

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Share to Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Source

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Identifiers

PMID: 22474337
DOI: 10.1073/pnas.1121471109
PMCID: PMC3341040

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