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Article

Serum-free culture of porcine and rabbit corneal epithelial cells.

Current eye research - 1 Sept 1990

Hackworth L A, Faraji-Shadan F, Schuschereba S T, Bowman P D

Abstract excerpt

To better define the growth requirements for corneal epithelial cells, methods for serum-free culture were established. Sheets of corneal epithelium from eyes of swine and rabbits were obtained by dispase treatment of corneal buttons, and single cells and small clumps were obtained by further dissociation with trypsin/EDTA. Growth of cells with epithelial-like morphology was readily achieved in low calcium MCDB...

Topics

Animals
Cell Division
Cells, Cultured
Cornea
Culture Media
Epithelial Cells
Epithelium
Phenotype
Rabbits
Swine

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2245650
DOI: 10.3109/02713689008999564

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2245650-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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