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Article

Diversity measures in environmental sequences are highly dependent on alignment quality--data from ITS and new LSU primers targeting basidiomycetes.

PloS one - 1 Jan 2012

Krüger Dirk, Kapturska Danuta, Fischer Christiane, Daniel Rolf, Wubet Tesfaye

Abstract excerpt

The ribosomal DNA comprised of the ITS1-5.8S-ITS2 regions is widely used as a fungal marker in molecular ecology and systematics but cannot be aligned with confidence across genetically distant taxa. In order to study the diversity of Agaricomycotina in forest soils, we designed primers targeting the more alignable 28S (LSU) gene, which should be more useful for phylogenetic analyses of the detected taxa. This...

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Identifiers

PMID: 22363808
DOI: 10.1371/journal.pone.0032139
PMCID: PMC3283731

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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