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Article

Fungal community analysis by large-scale sequencing of environmental samples.

Applied and environmental microbiology - 1 Sept 2005

O'Brien Heath E, Parrent Jeri Lynn, Jackson Jason A, Moncalvo Jean-Marc, Vilgalys Rytas

Abstract excerpt

Fungi are an important and diverse component of soil communities, but these communities have proven difficult to study in conventional biotic surveys. We evaluated soil fungal diversity at two sites in a temperate forest using direct isolation of small-subunit and internal transcribed spacer (ITS) rRNA genes by PCR and high-throughput sequencing of cloned fragments. We identified 412 sequence types from 863...

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Identifiers

PMID: 16151147
DOI: 10.1128/AEM.71.9.5544-5550.2005
PMCID: PMC1214672

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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