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The influence of intraspecific sequence variation during DNA metabarcoding: A case study of eleven fungal species.

Molecular ecology resources - 1 May 2021

Estensmo Eva Lena F, Maurice Sundy, Morgado Luis, Martin-Sanchez Pedro M, Skrede Inger, Kauserud Håvard

Abstract excerpt

DNA metabarcoding has become a powerful approach for analysing complex communities from environmental samples, but there are still methodological challenges limiting its full potential. While conserved DNA markers, like 16S and 18S, often are not able to discriminate among closely related species, other more variable markers - like the fungal ITS region, may include considerable intraspecific variation, which can...

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PMID: 33459491
DOI: 10.1111/1755-0998.13329

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