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Article

Semi-quantitative discrimination of HBV mutants using allele-specific oligonucleotide hybridization with Handy Bio-Strand.

Journal of bioscience and bioengineering - 1 Jan 2010

Ginya Harumi, Asahina Junko, Nakao Rumiko, Tamada Yohko, Takahashi Masaaki, Yohda Masafumi, Yatsuhashi Hiroshi

Abstract excerpt

The analysis of hepatitis B virus (HBV) mutations is important for understanding HBV progression and for deciding on appropriate clinical treatments. However, it is difficult to determine the quantitative abundance of various mutants in heterogeneous mixtures by conventional methods such as direct sequencing or the TaqMan assay. In this study, we investigated the possibility of using both allele-specific...

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Identifiers

PMID: 20129090
DOI: 10.1016/j.jbiosc.2009.06.023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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