Article
[Mechanism of loss of human esophageal cancer-related gene 4 (ECRG4) gene expression in esophageal squamous cell carcinoma cell line EC9706].
Zhonghua zhong liu za zhi [Chinese journal of oncology] - 1 Aug 2011
Li Lin-Wei, Yu Xi-Ying, Li Xiao-Yan, Guo Li-Ping, Zhou Yun, Lu Shi-Xin
Abstract excerpt
OBJECTIVE: To investigate the mechanism of loss of human esophageal cancer-related gene 4 (ECRG4) expression in esophageal squamous cell carcinoma (ESCC.) METHODS: PCR-SSCP and DNA sequencing analysis were used to detect the mutation of ECRG4 exons in esophageal cancer and matched adjacent normal tissues of 80 patients. DNA bisulfite-modifying ssPCR sequencing assay was used to examine the methylation status of...
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