Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Two-round allele specific-polymerase chain reaction: a simple and highly sensitive method for JAK2V617F mutation detection.

Clinica chimica acta; international journal of clinical chemistry - 1 Mar 2009

Kannim Supattra, Thongnoppakhun Wanna, Auewarakul Chirayu U

Abstract excerpt

BACKGROUND: Philadelphia chromosome (Ph(1))-negative myeloproliferative disorders (MPD) are hematopoietic stem cell disorders characterized by extensive proliferation of myeloid blood cells. JAK2V617F has recently been identified in the majority of Ph(1)-negative MPD and becomes an essential diagnostic marker. METHODS: To screen for JAK2V617F, a two-round allele specific-polymerase chain reaction (AS-PCR) was...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromatography, High Pressure Liquid
Share to DNA Mutational Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Early Detection of Cancer

Share to Janus Kinase 2

Share to Myeloproliferative Disorders

Share to Polymerase Chain Reaction

Share to Polymorphism, Restriction Fragment Length

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 19135044
DOI: 10.1016/j.cca.2008.12.010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Two-round allele specific-polymerase chain reaction: a simple and highly sensitive method for JAK2V617F mutation detection.PMID 19135044Read ↗

Development of a highly sensitive method for detection of JAK2V617F.Semantic proximity Read ↗

A novel, highly sensitive and rapid allele-specific loop-mediated amplification assay for the detection of the JAK2V617F mutation in chronic myeloproliferative neoplasms.Semantic proximity Read ↗

[The investigation of JAK2 V617F point mutation in myeloproliferative disorders by allele-specific polymerase chain reaction in combination with sequence analysis].Semantic proximity Read ↗

Study on the Clinical Significance of JAK2V617F Allele Burden in Philadelphia Chromosome-Negative Myeloproliferative Neoplasm.Semantic proximity Read ↗

Relevance of JAK2V617F positivity to hematological diseases--survey of samples from a clinical genetics laboratory.Semantic proximity Read ↗

The JAK2 V617F mutation involves B- and T-lymphocyte lineages in a subgroup of patients with Philadelphia-chromosome negative chronic myeloproliferative disorders.Semantic proximity Read ↗

Comparative study of different methodologies to detect the JAK2 V617F mutation in chronic BCR-ABL1 negative myeloproliferative neoplasmsSemantic proximity Read ↗

Development of an in-house real time q-PCR assay for the detection of jak2 mutations in myeloproliferative neoplasmsSemantic proximity Read ↗

JAK2V617F allele burden in patients with myeloproliferative neoplasms.Semantic proximity Read ↗

A comparison of qPCR and ddPCR used for quantification of the JAK2 V617F allele burden in Ph negative MPNs.Semantic proximity Read ↗

Design and evaluation of a real-time PCR assay for quantification of JAK2 V617F and wild-type JAK2 transcript levels in the clinical laboratory.Semantic proximity Read ↗

Real-Time PCR and Droplet Digital PCR: two techniques for detection of the JAK2(V617F) mutation in Philadelphia-negative chronic myeloproliferative neoplasms.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.