Article
Construction of a cellulase hyper-expression system in Trichoderma reesei by promoter and enzyme engineering.
Microbial cell factories - 8 Feb 2012
Zou Gen, Shi Shaohua, Jiang Yanping, van den Brink Joost, de Vries Ronald P, Chen Ling, Zhang Jun, Ma Liang, Wang Chengshu, Zhou Zhihua
Abstract excerpt
BACKGROUND: Trichoderma reesei is the preferred organism for producing industrial cellulases. However, a more efficient heterologous expression system for enzymes from different organism is needed to further improve its cellulase mixture. The strong cbh1 promoter of T. reesei is frequently used in heterologous expression, however, the carbon catabolite repressor CREI may reduce its strength by binding to the cbh1...
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