Article
Enhancing Cellulase and Hemicellulase Production in Trichoderma orientalis EU7-22 via Knockout of the creA.
Molecular biotechnology - 1 Jan 2018
Long Chuannan, Cheng Yijin, Cui Jingjing, Liu Jian, Gan Lihui, Zeng Bin, Long Minnan
Abstract excerpt
The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orientalis CF1D. For strain CF1D, the filter paper activities (FPA), endoglucanase activities (CMC),...
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