Article
Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger.
Biotechnology letters - 1 Jan 2016
Jiang Yanping, Duarte Alexandra Vivas, van den Brink Joost, Wiebenga Ad, Zou Gen, Wang Chengshu, de Vries Ronald P, Zhou Zhihua, Benoit Isabelle
Abstract excerpt
OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals. RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3...
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