The Copper-Controlled RNA Interference System in Trichoderma reesei.

doi:10.1007/978-1-0716-1048-0_9

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Article

The Copper-Controlled RNA Interference System in Trichoderma reesei.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Wang Lei, Zhang Weixin, Meng Xiangfeng, Liu Weifeng

Abstract excerpt

Trichoderma reesei is capable of secreting large amounts of lignocellulose-degrading enzymes. Although the genome sequence of T. reesei has been available, the molecular mechanisms of the hyper-production of cellulases, including the transcriptional regulation and the protein secretion, have not been completely elucidated yet. This is partially due to the lack of genetic manipulation approaches. RNA interference...

Topics

Copper
DNA, Fungal
Electrophoresis, Polyacrylamide Gel
Fermentation
Hypocreales
Phenotype
Plasmids
RNA Interference
RNA, Messenger
Transformation, Genetic

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PMID: 33165783
DOI: 10.1007/978-1-0716-1048-0_9

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