Article
Co-expression of Beta-Glucosidase and Laccase in Trichoderma reesei by Random Insertion with Enhanced Filter Paper Activity.
Molecular biotechnology - 1 Aug 2017
Wang Ruowen, Yang Jing, Zhang Guoqing, Chao Yapeng, Li Zhimin, Ye Qin, Qian Shijun
Abstract excerpt
Trichoderma reesei strain Rut-C30 was modified with enhanced beta-glycosidase (BGL) activity to balance the cellulase system and generated laccase (LAC) protein for lignin degradation. Initially, the binary plasmid p1300-w1 was constructed to express T. reesei bgl2 under the control of promoter P pki and T-nos terminator. Random insertion was performed via Agrobacterium tumefaciens-mediated transformation. A...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
