Article
Development of EST-SSR markers for Elaeocarpus photiniifolia (Elaeocarpaceae), an endemic taxon of the Bonin Islands.
American journal of botany - 1 Feb 2012
Sugai Kyoko, Setsuko Suzuki, Uchiyama Kentaro, Murakami Noriaki, Kato Hidetoshi, Yoshimaru Hiroshi
Abstract excerpt
PREMISE OF THE STUDY: Expressed sequence tag (EST)-derived microsatellite markers were developed for Elaeocarpus photiniifolia, an endemic taxon of the Bonin Islands. METHODS AND RESULTS: Initially, a complementary DNA (cDNA) library was constructed by de novo pyrosequencing of total RNA extracted from a seedling. A total of 267 primer pairs were designed from the library. Of the 48 tested loci, 25 loci were...
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