Article
Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags.
American journal of botany - 1 Jan 2012
Setsuko Suzuki, Uchiyama Kentaro, Sugai Kyoko, Hanaoka So, Yoshimaru Hiroshi
Abstract excerpt
PREMISE OF THE STUDY: Robust markers are required (inter alia) for assessing origins of Calophyllum inophyllum populations on the Bonin Islands, Japan. Therefore, informative expressed sequence tag (EST)-based microsatellite or simple sequence repeat (SSRs) markers in the species were sought. METHODS AND RESULTS: Using 135378 ESTs derived from de novo pyrosequencing, primers for 475 EST-SSRs were developed, 48 of...
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