Article
Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae).
American journal of botany - 1 Dec 2011
Sakaguchi Shota, Uchiyama Kentaro, Ueno Saneyoshi, Ujino-Ihara Tokuko, Tsumura Yoshihiko, Prior Lynda D, Bowman David M J S, Crisp Michael D, Isagi Yuji
Abstract excerpt
PREMISE OF THE STUDY: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. METHODS AND RESULTS: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence...
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