Expression of human chromosomal proteins HMG-14 and HMG-17 in Saccharomyces cerevisiae.

doi:10.1016/0014-4827(90)90037-b

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Article

Expression of human chromosomal proteins HMG-14 and HMG-17 in Saccharomyces cerevisiae.

Experimental cell research - 1 Nov 1990

Srikantha T, Dhar R, Bustin M

Abstract excerpt

The cDNAs coding for human chromosomal proteins HMG-14 and HMG-17 were cloned into yeast expression vector pBM150, under the control of the Gal10 promoter. Northern analysis of transformed yeast cells revealed that both cDNAs were efficiently transcribed. Western analysis indicated that the mRNAs...

Topics

Blotting, Northern
Blotting, Western
Chromosomal Proteins, Non-Histone
Cloning, Molecular
Electrophoresis, Gel, Two-Dimensional
Gene Expression
Humans
Phenotype
Plasmids
RNA, Messenger
Saccharomyces cerevisiae
Transfection

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