Article
Development and mapping of SNP assays in allotetraploid cotton.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 May 2012
Byers Robert L, Harker David B, Yourstone Scott M, Maughan Peter J, Udall Joshua A
Abstract excerpt
A narrow germplasm base and a complex allotetraploid genome have made the discovery of single nucleotide polymorphism (SNP) markers difficult in cotton (Gossypium hirsutum). To generate sequence for SNP discovery, we conducted a genome reduction experiment (EcoRI, BafI double digest, followed by adapter ligation, biotin-streptavidin purification, and agarose gel separation) on two accessions of G. hirsutum and...
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