Article
Non-viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation.
Molecular reproduction and development - 1 Apr 2012
Zeng W, Tang L, Bondareva A, Luo J, Megee S O, Modelski M, Blash S, Melican D T, Destrempes M M, Overton S A, Gavin W G, Ayres S, Echelard Y, Dobrinski I
Abstract excerpt
Germline stem cells (GSCs) can be used for large animal transgenesis, in which GSCs that are genetically manipulated in vitro are transplanted into a recipient testis to generate donor-derived transgenic sperm. The objectives of this study were to explore a non-viral approach for transgene delivery into goat GSCs and to investigate the efficiency of nucleofection in producing transgenic sperm. Four recipient...
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